gearray q human apoptosis gene array  (SuperArray Bioscience Corporation)

Journal:

Article Title: Immunoresistant human glioma cell clones selected with alloreactive cytotoxic T lymphocytes

doi:

Figure Lengend Snippet: Apoptosis of parental glioma cells and IR clones, detected by an in vitro quantitative morphologic assay, increases upon their coincubation with aCTL. Monolayer cultures were washed after coincubation with aCTL at a 25:1 E:T ratio for 4 hr. After fixing, the adherent glioma cells were stained with hematoxylin and eosin. The percentages of apoptotic glioma cells ± standard deviation were determined from a total of 800 cell counts (400 cell counts/glioma cell type/experiment) taken under high-power microscopy.

Article Snippet: The gene expression profiles of parental and IR glioma cells were analyzed using the GEArray Q Series Human Apoptosis Gene Arrays (SuperArray Bioscience Corp., Frederick, MD).

Techniques: Clone Assay, In Vitro, Staining, Standard Deviation, Microscopy

Journal:

Article Title: Immunoresistant human glioma cell clones selected with alloreactive cytotoxic T lymphocytes

doi:

Figure Lengend Snippet: Apoptosis pathway specific microarrays of parental 13-06-MG glioma cells and related IR glioma clones, along with identification of differentially expressed apoptosis genes associated with the IR phenotype. Total RNA (5 μg) from aCTL -sensitive parental and aCTL-resistant glioma clones was isolated, reverse transcribed and the resulting cDNA hybridized to apoptosis pathway specific arrays. (a) Inverted images show hybridization signals on the apoptosis arrays from the parental and IR cDNA. The location of the negative controls and housekeeping genes (HKG) are in the two bottom rows. The apoptotic protease activating factor -1 (Apaf-1), the ataxia telangiectasia mutated (ATM), the caspase 3 and caspase 8 genes are shown in boxes at column/row positions, going left to right, for each array at 1,1; 1,3; 4,4, and 1,5). (b) Differentially expressed apoptosis associated genes in common to both IR clones at ≥ 2 fold differences compared to the parental glioma cells, as determined with the GEArray Analyzer software program. An asterisk indicates that a particular gene was not expressed.

Article Snippet: The gene expression profiles of parental and IR glioma cells were analyzed using the GEArray Q Series Human Apoptosis Gene Arrays (SuperArray Bioscience Corp., Frederick, MD).

Techniques: Clone Assay, Isolation, Reverse Transcription, Hybridization, Software